{"created":"2023-06-20T13:45:29.200683+00:00","id":254,"links":{},"metadata":{"_buckets":{"deposit":"a5d4c06e-f7ab-4747-8512-00bb8fdcaddd"},"_deposit":{"created_by":12,"id":"254","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"254"},"status":"published"},"_oai":{"id":"oai:khsu.repo.nii.ac.jp:00000254","sets":["71"]},"author_link":["424","425"],"item_10007_contributor_5":{"attribute_name":"研究分担者","attribute_value_mlt":[{"contributorNames":[{"contributorName":"工楽, 樹洋"}],"nameIdentifiers":[{"nameIdentifier":"425","nameIdentifierScheme":"WEKO"}]}]},"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017","subitem_date_issued_type":"Issued"}]},"item_10007_description_10":{"attribute_name":"研究代表者番号","attribute_value_mlt":[{"subitem_description":"90109637","subitem_description_type":"Other"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要：我々は、樹立したマウス精巣の再凝集培養系を用いて、精巣形成に関わるシグナリング因子、間細胞の役割等について、Live imaging等を活用しながら解析した。その結果、CD34+細胞、p75+細胞、筋様細胞は内側から外側にかけて重層構造を形成して間組織を再構築すること、精細管様構造の再構築に間細胞が必要であること、ALK5i(ALK5阻害剤)は間細胞の増殖や細胞運動を阻害すること、純化したCD34+細胞を培養するとp75+細胞を経て筋様細胞へ分化することを示した。これらの結果は、間組織と精細管様構造の再構築にCD34+細胞とALK5シグナリングが重要であることを示唆する。","subitem_description_type":"Abstract"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"15K07129","subitem_description_type":"Other"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-08"}],"displaytype":"detail","filename":"kaken.nii.ac.jp_15K07129seika.pdf","filesize":[{"value":"842.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kaken.nii.ac.jp_15K07129seika","url":"https://khsu.repo.nii.ac.jp/record/254/files/kaken.nii.ac.jp_15K07129seika.pdf"},"version_id":"ad0378a5-f9ca-4845-8691-de588570d7a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マウス精巣","subitem_subject_scheme":"Other"},{"subitem_subject":"３次元培養","subitem_subject_scheme":"Other"},{"subitem_subject":"精細管","subitem_subject_scheme":"Other"},{"subitem_subject":"再構築","subitem_subject_scheme":"Other"},{"subitem_subject":"KSR","subitem_subject_scheme":"Other"},{"subitem_subject":"ALK5","subitem_subject_scheme":"Other"},{"subitem_subject":"Live Imaging","subitem_subject_scheme":"Other"},{"subitem_subject":"CD34+細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"p75+細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"筋様細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"再凝集培養","subitem_subject_scheme":"Other"},{"subitem_subject":"Alk5","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安部 , 眞一"}],"nameIdentifiers":[{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"３次元培養系による精細管構造の再構築メカニズム","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"３次元培養系による精細管構造の再構築メカニズム"},{"subitem_title":"Mechanisms underlying reconstruction of seminiferous tubule structure by 3-D culture system","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"12","path":["71"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-08"},"publish_date":"2022-03-08","publish_status":"0","recid":"254","relation_version_is_last":true,"title":["３次元培養系による精細管構造の再構築メカニズム"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-06-20T13:49:02.042238+00:00"}